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A simple, selective, accurate high performance liquid chromatographic method was developed and validated for an analysis of Halobetasol Propionate and p-Chlorocresol in pharmaceutical formulations. Chromatographic separation achieved by gradient programme on a C18 column (Waters Novapak C-18, 250 x 4.6mm, 4µm column) utilizing a mobile phases of A (pH 2.5 phosphate buffer) and B (mixture of buffer, acetonitrile and methanol in the ratio of 20:70:10, v/v/v respectively) at a flow rate of 1.0 ml/min with UV detection at 240nm. The retention time of Halobetasol Propionate and p-Chlorocresol were6.3 and 8.9 min, respectively. The total run time is 15 minutes. The developed method was validated in terms of accuracy, precision, linearity, specificity, robustness and solution stability.This study aimed at developing and validating an HPLC method, being simple, accurate and selective and the proposed method can be used for the estimation of these two components in pharmaceutical preparations.


Halobetasol Propionate p-Chlorocresol RP-HPLC Simultaneous determination Method validation

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How to Cite
Lakka, N. S., & Goswami , N. (2012). A STABILITY INDICATING RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF HALOBETASOL PROPIONATE AND P-CHLOROCRESOL IN CREAMS . International Research Journal of Pharmaceutical and Applied Sciences, 2(5), 13-19. Retrieved from