A SIMPLE AND VALIDATED RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF RABEPRAZOLE AND LEVOSULPIRIDE IN BULK AND PHARMACEUTICAL DOSAGE FORMS

Abstract

A simple, rapid, specific, accurate and precise reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of Rabeprazole and Levosulpiride in Tabletdosage form. A Thermo Hypersil ODS C18 5mm column having 250 x 4.6mm id in Isocratic mode with mobile phase containing 0.1M Ammonium Acetate : methanol (50:50 %v/v pH: 7.0) was used. The flow rate was 1.5ml/min and effluents were monitored at 290nm. The retention time of Levosulpiride and Rabeprazole was 2.2min and 6.8min respectively. The concentration curves were linear in the concentration range of 5-15 µg/mL and18.75-56.25 µg/mL. The developed method was validated for specificity, precision, linearity, accuracy, LOD,LOQ, robustness . Recovery of Rabeprazole and Levosulpiride in formulations was found to be in the range of 98.0% -103.0% and 99%-103% respectively confirms the non-interferences of the excipients in the formulation. Due to its simplicity, rapidness and high precision, the proposed HPLC method may be used for the simultaneous determination of these two drugs in pharmaceutical dosage forms